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Image Search Results
Journal: Investigative Ophthalmology & Visual Science
Article Title: Myeloid Suppressor Cells Induced by Retinal Pigment Epithelial Cells Inhibit Autoreactive T-Cell Responses That Lead to Experimental Autoimmune Uveitis
doi: 10.1167/iovs.11-8377
Figure Lengend Snippet: RPE cell–induced MDSCs inhibited T-cell responses. Spleen cells (5 × 105) from naive C57BL/6 mice were labeled with CFSE and activated by anti–CD3 mAb, then cocultured with different numbers of the RPE cell–induced MDSCs. In 2 days, the inhibition of T-cell responses was assessed by evaluating proliferating T cell–formed clusters directly under a microscope (A, top) and by measuring CFSE dilution using flow cytometry, gating on the CD4+ T cells (A, bottom). The inhibition of T-cell responses (B) was calculated using the following formula: inhibition (%) = 1 − [(b − a)/a], where a is the number of proliferating T cells without MDSCs and b is the number of proliferating T cells with MDSCs. IFN-γ levels in the supernatants were measured by standard ELISA (C).
Article Snippet: Then IFN-γ and IL-17 levels in the supernatants were measured using
Techniques: Labeling, Inhibition, Microscopy, Flow Cytometry, Enzyme-linked Immunosorbent Assay
Journal: Investigative Ophthalmology & Visual Science
Article Title: Myeloid Suppressor Cells Induced by Retinal Pigment Epithelial Cells Inhibit Autoreactive T-Cell Responses That Lead to Experimental Autoimmune Uveitis
doi: 10.1167/iovs.11-8377
Figure Lengend Snippet: RPE cell–induced MDSCs suppress in vivo autoreactive T-cell responses that lead to retinal injury in EAU. RPE cell–induced MDSCs (2 × 106) in 0.5 mL PBS were adoptively transferred into each of the six female C57BL/6 mice after EAU induction by tail vein intravenous injection. The same volume of PBS was administered into each of the six control mice. In 21 days, EAU disease severity was evaluated by histopathologic scoring of the ocular sections in a masked fashion. Each dot represents one eye (A). Shown are representative images of the retina from the mock-treated and MDSC-treated mice (B). IRBP-specific Th1 and Th17 cell responses in the experimental mice were assessed by IFN-γ and IL-17 ELISA using culture supernatants collected from splenocytes restimulated with different concentrations of the IRBP1–20 peptide (C).
Article Snippet: Then IFN-γ and IL-17 levels in the supernatants were measured using
Techniques: In Vivo, Injection, Enzyme-linked Immunosorbent Assay