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90
DYNEX tech all conventional elisa kits
All Conventional Elisa Kits, supplied by DYNEX tech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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all conventional elisa kits - by Bioz Stars, 2026-03
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Becton Dickinson conventional elisa kits
RPE cell–induced MDSCs inhibited T-cell responses. Spleen cells (5 × 105) from naive C57BL/6 mice were labeled with CFSE and activated by anti–CD3 mAb, then cocultured with different numbers of the RPE cell–induced MDSCs. In 2 days, the inhibition of T-cell responses was assessed by evaluating proliferating T cell–formed clusters directly under a microscope (A, top) and by measuring CFSE dilution using flow cytometry, gating on the CD4+ T cells (A, bottom). The inhibition of T-cell responses (B) was calculated using the following formula: inhibition (%) = 1 − [(b − a)/a], where a is the number of proliferating T cells without MDSCs and b is the number of proliferating T cells with <t>MDSCs.</t> <t>IFN-γ</t> levels in the supernatants were measured by standard <t>ELISA</t> (C).
Conventional Elisa Kits, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/conventional elisa kits/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
conventional elisa kits - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

90
FineTest Biotech Inc conventional elisa kits
RPE cell–induced MDSCs inhibited T-cell responses. Spleen cells (5 × 105) from naive C57BL/6 mice were labeled with CFSE and activated by anti–CD3 mAb, then cocultured with different numbers of the RPE cell–induced MDSCs. In 2 days, the inhibition of T-cell responses was assessed by evaluating proliferating T cell–formed clusters directly under a microscope (A, top) and by measuring CFSE dilution using flow cytometry, gating on the CD4+ T cells (A, bottom). The inhibition of T-cell responses (B) was calculated using the following formula: inhibition (%) = 1 − [(b − a)/a], where a is the number of proliferating T cells without MDSCs and b is the number of proliferating T cells with <t>MDSCs.</t> <t>IFN-γ</t> levels in the supernatants were measured by standard <t>ELISA</t> (C).
Conventional Elisa Kits, supplied by FineTest Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/conventional elisa kits/product/FineTest Biotech Inc
Average 90 stars, based on 1 article reviews
conventional elisa kits - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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RPE cell–induced MDSCs inhibited T-cell responses. Spleen cells (5 × 105) from naive C57BL/6 mice were labeled with CFSE and activated by anti–CD3 mAb, then cocultured with different numbers of the RPE cell–induced MDSCs. In 2 days, the inhibition of T-cell responses was assessed by evaluating proliferating T cell–formed clusters directly under a microscope (A, top) and by measuring CFSE dilution using flow cytometry, gating on the CD4+ T cells (A, bottom). The inhibition of T-cell responses (B) was calculated using the following formula: inhibition (%) = 1 − [(b − a)/a], where a is the number of proliferating T cells without MDSCs and b is the number of proliferating T cells with MDSCs. IFN-γ levels in the supernatants were measured by standard ELISA (C).

Journal: Investigative Ophthalmology & Visual Science

Article Title: Myeloid Suppressor Cells Induced by Retinal Pigment Epithelial Cells Inhibit Autoreactive T-Cell Responses That Lead to Experimental Autoimmune Uveitis

doi: 10.1167/iovs.11-8377

Figure Lengend Snippet: RPE cell–induced MDSCs inhibited T-cell responses. Spleen cells (5 × 105) from naive C57BL/6 mice were labeled with CFSE and activated by anti–CD3 mAb, then cocultured with different numbers of the RPE cell–induced MDSCs. In 2 days, the inhibition of T-cell responses was assessed by evaluating proliferating T cell–formed clusters directly under a microscope (A, top) and by measuring CFSE dilution using flow cytometry, gating on the CD4+ T cells (A, bottom). The inhibition of T-cell responses (B) was calculated using the following formula: inhibition (%) = 1 − [(b − a)/a], where a is the number of proliferating T cells without MDSCs and b is the number of proliferating T cells with MDSCs. IFN-γ levels in the supernatants were measured by standard ELISA (C).

Article Snippet: Then IFN-γ and IL-17 levels in the supernatants were measured using conventional ELISA kits (BD Biosciences).

Techniques: Labeling, Inhibition, Microscopy, Flow Cytometry, Enzyme-linked Immunosorbent Assay

RPE cell–induced MDSCs suppress in vivo autoreactive T-cell responses that lead to retinal injury in EAU. RPE cell–induced MDSCs (2 × 106) in 0.5 mL PBS were adoptively transferred into each of the six female C57BL/6 mice after EAU induction by tail vein intravenous injection. The same volume of PBS was administered into each of the six control mice. In 21 days, EAU disease severity was evaluated by histopathologic scoring of the ocular sections in a masked fashion. Each dot represents one eye (A). Shown are representative images of the retina from the mock-treated and MDSC-treated mice (B). IRBP-specific Th1 and Th17 cell responses in the experimental mice were assessed by IFN-γ and IL-17 ELISA using culture supernatants collected from splenocytes restimulated with different concentrations of the IRBP1–20 peptide (C).

Journal: Investigative Ophthalmology & Visual Science

Article Title: Myeloid Suppressor Cells Induced by Retinal Pigment Epithelial Cells Inhibit Autoreactive T-Cell Responses That Lead to Experimental Autoimmune Uveitis

doi: 10.1167/iovs.11-8377

Figure Lengend Snippet: RPE cell–induced MDSCs suppress in vivo autoreactive T-cell responses that lead to retinal injury in EAU. RPE cell–induced MDSCs (2 × 106) in 0.5 mL PBS were adoptively transferred into each of the six female C57BL/6 mice after EAU induction by tail vein intravenous injection. The same volume of PBS was administered into each of the six control mice. In 21 days, EAU disease severity was evaluated by histopathologic scoring of the ocular sections in a masked fashion. Each dot represents one eye (A). Shown are representative images of the retina from the mock-treated and MDSC-treated mice (B). IRBP-specific Th1 and Th17 cell responses in the experimental mice were assessed by IFN-γ and IL-17 ELISA using culture supernatants collected from splenocytes restimulated with different concentrations of the IRBP1–20 peptide (C).

Article Snippet: Then IFN-γ and IL-17 levels in the supernatants were measured using conventional ELISA kits (BD Biosciences).

Techniques: In Vivo, Injection, Enzyme-linked Immunosorbent Assay